Dr. Heimo Wolinski (Senior Scientist)
phone: +43 (316) 380 - 5489
Yeast and mammalian live cell imaging.
Confocal and multi-photon microscopy.
Development of multi-dimensional visualization and analysis techniques for yeast applications.
Evaluation of preparations techniques for yeast live cell imaging.
Application of advanced imaging techniques and novel GFP technologies in yeast and mammalian cells: 4D imaging, CARS, SHG, FRAP, FRET, PA-GFP.
Investigating localization, interactions and dynamics of proteins involved in lipid metabolism and lipid droplet biogenesis.
Project: SFB, GOLD
Full name: Heimo Wolinski.
Date of birth: August 5, 1970.
Studies, Karl-Franzens-University Graz, Institute of Botany.
Diploma thesis at Institute of Botany, Karl-Franzens-University Graz. Title: "Microslide technique and application on fungal material: Direct PCR, in situ hybridization and automation".
1999-2000: Civil service.
Doctoral thesis at Institute of Botany, Karl-Franzens-University Graz. Title: "Visualization of fungal nuclear structures in complex cell systems".
Research fellowship of the Natural Faculty of Science, University Graz.
January 2003- :
Postdoctoral position at SFB Biomembrane Research Center, Institute of Molecular Biology, Biochemistry and Microbiology (IMB-Biochemistry), Karl-Franzens-University Graz, group of Prof. Kohlwein.
Specific project at the institute: “Spatial organization of lipid synthesis in yeast”. This project deals with the analysis of the distribution of enzymes involved in lipid synthesis in various subcellular organelles, and their localization in response to changes in lipid composition.
Klaus Natter, Peter Leitner, Alexander Faschinger, Heimo Wolinski, Stephen McCraith, Stanley Fields and Sepp D. Kohlwein (2005). The spatial organization of lipid synthesis in the yeast Saccharomyces cerevisiae derived from large-scale green fluorescent protein tagging and high-resolution microscopy. Mol Cell Proteomics 4(5):662-672.
Wolinski, H, Heise, B, Klement, E.P., Sonnleitner, A., Kohlwein, S.D. (2005). Efficient quantitative analysis of fluorescently labelled thee-dimensional subcellular yeast structures. In prep.
Wolinski, H (2003). Confocal imaging reveals structural detail of the cell nucleus and ascospore formation in lichenized fungi. Mycological Research 107: 989-995.
Lukas M, Rosker C, Wolinski H, Kohlwein S, Groschner K (2003). Characterization of lipid raft domains which sequester TRPC3 in the plasma membrane of HEK293 cells. Biophysical Journal 84.
Lukas M, Rosker C, Wolinski H, Kohlwein SD, Groschner K (2003). TRPC3 function is dependent on the structure of cholesterol-rich microdomains. Pharmacology 69.
Wolinski H (2002). Visualization of fungal nuclear structures in complex cell systems. Dissertation. Institute of Botany, Karl-Franzens-University Graz.
Wolinski, H, Grube, M, Blanz P (1999). Direct PCR of symbiotic fungi using microslides. Biotechniques 26: 454-455.
Wolinski, H. (2005). Talk - Efficient visualization and analysis of fluorescently labelled subcellular structures of living yeast cells. International Botanical Congress, Vienna.
Wolinski, H, Heise, B, Klement EP, Sonnleitner, A, Kohlwein, SD (2005). Efficient visualization and analysis of fluorescently labelled subcellular structures of living yeast cells. Focus on Microscopy, Jena, Germany. Abstract book.
Julia Petschnigg , Christoph Kurat, Regina Leber, Klaus Natter, Harald Scholz, Heimo Wolinski, Nermina Malanovic, Katrin Fantur, Astrid Gruber, Kathrin Winkler, Sepp D. Kohlwein. (2005). Neutral lipid homeostasis in the yeast Saccharomyces cerevisiae . XXIIth YGM Conference, Bratislava, Slovak Republic. Abstract book.
Uros Petrovic, Mojca Mattiazzi, Jernej Sribar, Heimo Wolinski, Harald Scholz, Sepp D. Kohlwein, Igor Krizaj. (2005). Cellular processes affected by a phospholipase A 2 in yeast. XXIIth YGM Conference, Bratislava, Slovak Republic. Abstract book.
Wolinski, H, Kohlwein, SD (2004). 4D imaging of living yeast cells: Analysing protein and organelle dynamics in vivo. European Light Microscopy Initiative Meeting Gothenburg, Sweden, Abstract book.
Wolinski, H, Kohlwein, SD (2004). 4D imaging of the living yeast cells: Analysing protein dynamics in vivo. Annual Linz Winter Workshop, Austria. Abstract book.
Wolinski, H, Kohlwein, SD (2004). 4D imaging of living yeast cells: Analysing protein and organelle dynamics in vivo. European Microscopy Congress Antwerp, Belgium. Abstract book.
Hasek, J, Janatova, I, Spryngar, M, Wolinski, H, Kohlwein, SD (2004). Rpg1p/eIF3a of Saccharomyces cerevisiae accumulates in stress granules. Yeast Genetics and Molecular Biology Meeting Seattle, USA. Abstract book.
M. Spryngar, I. Janatova, K. Malinska, H. Wolinski, S. D. Kohlwein, and J. Hasek. (2004). eIF3a/Rpg1p of Saccharomyces cerevisiae mediates formation of stress granules. American Society for Cell Biology, Washington DC, USA. Abstract book.
Pongratz, M, Hacker, M, Wolinski, H, Kohlwein, SD, Schulz, MB (2004). In vitro evaluation of the angiogenic potential of gel matrices with the „rat aortic ring model“. Strategies in Tissue Engineering Würzburg, Germany. Abstract book.
Merkel, O, Wolinski, H, Ulrich, A, Liuta-Teglivets, O, Dib, I, Rechberger, N, Kohlwein, SD (2004). Regulators of the Acetyl-CoA Carboxylase and their impact on lipid homeostasis in Saccharomyces cerevisiae. APP - First International Symposium, Innsbruck, Austria. Abstract book.
Natter, K, Dib, I, Leitner, P, Fields, St, McCraith, St, Wolinski, H, Kohlwein, SD (2003). The spatial organization of lipid synthesis in yeast. Yeast, Göteborg. Abstract book.
Wolinski, H, Kroneis, Th, Kohlwein, SD (2003). Procedure for 2D and 3D live cell imaging in the yeast. Yeast, ELMI meeting Göteborg. Abstract book.
Wolinski H, Grube M, Blanz, P (2003). Three-dimensional visualization of fungal nuclei in complex cell systems. Focus on Microscopy, Genova. Abstract book.